Following the plot of the movie, the game essentially acts as a companion to the film franchise and an extension of the universe. After being bitten by a genetically enhanced Spider whilst on a field trip with his school, Peter Parker starts to develop abnormal abilities reflecting those of spiders. Not long afterwards, Peter is witness to the death of his Uncle Ben and immediately uses his new found abilities to chase down the track down the man responsible. After a brief interrogation, Peter learns that it was a member of a local gang, the Skulls that did it and Peter immediately chases the man down to the Skull ganghouse by the port. After busting in and beating down everyone willing to cross his path, Peter battles with the man who killed his uncle, which quickly escalates and leads to the murderers' death. Feeling responsible for his Uncles death, Peter takes on board one of the last thing Ben Parker said to him: With great power, comes great responsibility. Taking this on board, Peter becomes Spider-Man and vows to use his powers for good.
A few months later, we find Spider-Man battling the scum of Manhattan, and taking pictures of himself so that as Peter Parker, he can keep his job working as a freelance photographer for The Daily Bugle, a local newspaper that has a habit of criminalising Spider-Man. Meanwhile, at a nearby Bank, a bunch of goons and Shocker are driving away from their heist. As Spidey lands, he is briefly attacked by the Vulture. Spidey chooses to chase down Shocker first, engaging in an electric battle with him before Spidey emerges victorious. Spider-Man interrogates Shocker and finds that the Vulture is hiding out in a clock tower in the lower-east, and Spider sets off immediately. Once he finds Vulture, he finds he has to chase him throughout New York, a pursuit that ends in a battle all around the Chrysler building in which Spider-Man once again wins.
As Peter checks out the site where he fought Shocker for some pictures, he runs into the Scorpion who is being chased by small spider-like machines. After helping Scorpion destroy them all, the Scorpion becomes paranoid and turns on Spidey who battles reluctantly and wins once more.
Meanwhile Norman Osbourne, father to Peter's best friend Harry Osbourne, finds himself fired from his own company, Oscorp. Through desperation, he exposes himself to a potent and experimental new form of genome-gas, designed to make the human body stronger, faster and more reliable. The trial is a resounding success but the cost is Osbourne's mind which is transformed from the caring, if enthusiastic Norman Osbourne, to the homicidal and exaggerated Green Goblin. Stealing a glider and a flight suit from Oscorp, The Green Goblin begins his plans to take revenge on those who fired him from Oscorp.
Soon after that during Oscorp Unity Day Festival, The Green Goblin makes his attack. Killing many Oscorp executives, and attempting to kill many other innocent civilians (amongst them, the love of Peter's life, Mary Jane Watson) The Green Goblins assault is foiled by Spider-Man who shows up just in time,
It doesn't take The Green Goblin long to launch his next assault, as he sets up a number of bombs and sets a series of 'Razor Bat' weapons on Spider-Man who successfully defeats The Goblin and disarms all the bombs. Finding a defeated Razor Bat, Peter examines it and decides to check out Oscorp to try and make a connection between the arms company and The Green Goblin. Whilst there he manages to stop the threat of a chemical toxins leak. However, upon exiting Oscorp, he discovers that The Goblin has learned of his true identity and is taking Mary Jane hostage. Spider-Man chases The Green Goblin to a bridge where Spidey and The Goblin engage in their final battle. Eventually, Peter avoids The Goblins glider which then runs over the Goblin.
Seemingly regaining consciousness in his final moments, Norman remarks "Tell Harry I'm sorry." to which Spider-Man responds "I'm sorry too."
As Norman dies, Spider-Man kisses Mary Jane and the game ends.
The game is divided into sections with 3 levels in each section. Each section has it's own enemy and charts progress throughout the plot, so cannot be picked at will, unless sections have been unlocked. Much focus is placed on Spider-Man's abilities, such as web-slinging, zipping, firing webs at enemies, tying them up with web, crawling on walls and stringing together combo's that use these dynamics. Using these abilities can appear different, based on how you press the buttons, or in which order. Certain objects throughout the game can be lifted, and wall crawling is automatic throughout. There is a type of stealth mode in which the player cannot be seen if Spider-Man is hidden by shadows. As well as this, certain golden combo spiders can be found extending the players ability to pull combos.
Throughout the game there are bonuses you can collect consisting of:
You can gain points for these tasks, and depending on your difficulty, they can range from 500 points to 1000 points each. Through bonuses you can also unlock pinhead bowling.
After beating the game you will unlock the ability to play as the Green Goblin. This goblin is actually Harry Osbourne trying to get revenge on Spider man. The game levels stay the same, but voice actors and story elements change slightly. Goblin has the ability to fly hover and use various weapons on the glider. The glider is equiped with razors, machine guns and bombs. While the levels stay the same the game does feel very different while using Harry as the Goblin.
Spider-Man / Peter Parker...Tobey Maguire
Green Goblin / Norman Osborn....Willem Dafoe
Tour Guide...Bruce Campbell
Harry Osborn...Josh Keaton
Mary Jane...Catherine O'Connor
Adrian Toomes / Vulture...Dwight Schultz
Herman Shultz / Shocker...Michael Beattie
Mac Gargan / Scorpion...Mike McColl
J. Jonah Jameson...Jay Gordon
Sergei Kravinoff / Kraven the Hunter...Peter Lurie
Dennis "Spike" Carradine...Michael Papajohn
Senior Producer...Gregory John
Creative Director...Chris Soares
Lead Programmer...James Fristrom
Lead Game Designer...Tomo Moriwaki
Audio Direction...Sergio Bustamante the Second
Lead Engineer...Jason Bare
Lead Enviroment Modeler...Alex PBortoluzzi
Assistant Producer...Robert Sanches
Game Designer...Aki Akaike
Game Designer...Joel Burgess
Game Designer...Winnie Lee
Game Designer...Eric Pavone
Game Designer...Rey Samonte
Game Designer/Effects Artist...James Chao
Game Designer/Writer...Matthew B. Rhoades
Engineer/Platform Programmer...Greg Taylor
Tools/Front End Programmer...Bob Parkinson Jr.
Lead Animator...James Zachary
Character Modeler...Arnold Agraviador
Character Modeler...Miranda Collins
Character Modeler...Brad Grace
Character Modeler...Kevin Pasko
Character/Level Texture Artist...Michael McMahan
Cinematics Texture Artist...Dusty Peterson
Senior Sound Designer...Douglas Rappaport
Sound Designer...Kris Giampa